Industrial Methods of Analysis book, notes pdf - b pharmacy 4th year (7th semester) - JNTUH
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B Pharmacy 4th year (1st sem) | 7th Semester | JNTUH
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Unit -1
UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component analysis
Fluorimetry
Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications.
Unit - 2
IR spectroscopy
Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations.
Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermistor, Pyroelectric detector and applications
Flame Photometry - Principle, interferences, instrumentation and applications
Atomic absorption spectroscopy - Principle, interferences, instrumentation and applications
Nepheloturbidometry - Principle, instrumentation and applications
Unit - 3
Introduction to chromatography
Adsorption and partition column chromatography- Methodology, advantages, disadvantages and applications.
Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.
Paper chromatography- Introduction, methodology, development techniques, advantages, disadvantages and applications
Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications.
Unit - 4
Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications.
High performance liquid chromatography (HPLC) - Introduction, theory, instrumentation, advantages and applications.
Unit - 5
Ion exchange chromatography - Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications.
Gel chromatography - Introduction, theory, instrumentation and applications
Affinity chromatography - Introduction, theory, instrumentation and applications
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