Industrial Methods of Analysis book, notes pdf - b pharmacy 4th year (7th semester) - JNTUH

 Nano Technology

 Notes | Books | Syllabus | Previous Paper

B Pharmacy 4th year (1st sem) | 7th Semester | JNTUH



Unit -1

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component analysis

Fluorimetry

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications.


Unit - 2

IR spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations.

Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermistor, Pyroelectric detector and applications

Flame Photometry - Principle, interferences, instrumentation and applications

Atomic absorption spectroscopy - Principle, interferences, instrumentation and applications

Nepheloturbidometry - Principle, instrumentation and applications


Unit - 3

Introduction to chromatography

Adsorption and partition column chromatography- Methodology, advantages, disadvantages and applications.

Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.

Paper chromatography- Introduction, methodology, development techniques, advantages, disadvantages and applications

Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications.


Unit - 4

Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications.

High performance liquid chromatography (HPLC) - Introduction, theory, instrumentation, advantages and applications.


Unit - 5

Ion exchange chromatography - Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications.

Gel chromatography - Introduction, theory, instrumentation and applications

Affinity chromatography - Introduction, theory, instrumentation and applications






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